0435780 - ÚMG 2015 RIV GB eng J - Článek v odborném periodiku
Di Ruscio, A. - Ebralidze, A. - Benoukraf, T. - Amabile, G. - Goff, L.A. - Terragni, J. - Figueroa, M.E. - Pontes, L.L.D. - Alberich-Jorda, Meritxell - Zhang, P. - Wu, M.C. - D´Alo, F. - Melnick, A. - Leone, G. - Ebralidze, K.K. - Pradhan, S. - Rinn, J.L. - Tenen, D.G.
DNMT1-interacting RNAs block gene-specific DNA methylation.
Nature. Roč. 503, č. 7476 (2013), s. 371-376. ISSN 0028-0836. E-ISSN 1476-4687
Grant CEP: GA MŠMT LK21307
Institucionální podpora: RVO:68378050
Klíčová slova: DNA methylation * non-coding RNA * DNMT1
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 42.351, rok: 2013 ; AIS: 22.184, rok: 2013
DOI: https://doi.org/10.1038/nature12598

DNA methylation was first described almost a century ago; however, the rules governing its establishment and maintenance remain elusive. Here we present data demonstrating that active transcription regulates levels of genomic methylation. We identify a novel RNA arising from the CEBPA gene locus that is critical in regulating the local DNA methylation profile. This RNA binds to DNMT1 and prevents CEBPA gene locus methylation. Deep sequencing of transcripts associated with DNMT1 combined with genome-scale methylation and expression profiling extend the generality of this finding to numerous gene loci. Collectively, these results delineate the nature of DNMT1-RNA interactions and suggest strategies for gene-selective demethylation of therapeutic targets in human diseases.
Trvalý link: http://hdl.handle.net/11104/0239656