Počet záznamů: 1  

Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants

  1. 1. 0435644 - UEM-P 2015 RIV NL eng J - Článek v odborném periodiku
    Rössner ml., Pavel - Rössnerová, Andrea - Beskid, Olena - Tabashidze, Nana - Líbalová, Helena - Uhlířová, Kateřina - Topinka, Jan - Šrám, Radim
    Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants.
    Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 763-764, MAY-JUN 2014 (2014), s. 28-38 ISSN 0027-5107
    Grant CEP: GA ČR GAP503/11/0084
    Institucionální podpora: RVO:68378041
    Klíčová slova: benzo[a]pyrene * chromosome aberrations * double-strand DNA breaks
    Kód oboru RIV: DN - Vliv životního prostředí na zdraví
    Impakt faktor: 3.680, rok: 2014

    The cells were treated for 24 h with B[a]P (1 μM, 10 μM, 25 μM) and EOMs (1 μg/ml, 10 μg/ml, 25 μg/ml). 25 μM of B[a]P and most of the tested doses of EOMs induced double strand breaks (DSBs) as indicated by H2AX phosphorylation. DNA damage was accompanied by induction of XRCC4 expression and an increased frequency of chromosomal aberrations. Translocations most frequently affected chromosome 7. We observed only a weak induction of Ku70/80 expression as well as ligation activity of nuclear extracts.
    Trvalý link: http://hdl.handle.net/11104/0239451