0435557 - MBÚ 2015 RIV US eng J - Článek v odborném periodiku
Górska, S. - Schwarzer, Martin - Jachymek, W. - Šrůtková, Dagmar - Brzozowska, E. - Kozáková, Hana - Gamian, A.
Distinct Immunomodulation of Bone Marrow-Derived Dendritic Cell Responses to Lactobacillus plantarum WCFS1 by Two Different Polysaccharides Isolated from Lactobacillus rhamnosus LOCK 0900.
Applied and Environmental Microbiology. Roč. 80, č. 20 (2014), s. 6506-6516. ISSN 0099-2240. E-ISSN 1098-5336
Institucionální podpora: RVO:61388971
Klíčová slova: MAGNETIC-RESONANCE SPECTROSCOPY * ASSISTED STRUCTURAL-ANALYSIS * LACTIC-ACID BACTERIA
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.668, rok: 2014 ; AIS: 1.29, rok: 2014
DOI: https://doi.org/10.1128/AEM.02104-14

The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results from stimulation of mouse bone marrow-derived dendritic cells (BM-DC) and human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. rhamnosus LOCK 0900 produces PS that differ greatly in their structure. The polymer L900/2, with a high average molecular mass of 830 kDa, is a branched heteropolysaccharide with a unique repeating unit consisting of seven sugar residues and pyruvic acid, whereas L900/3 has a low average molecular mass of 18 kDa and contains a pentasaccharide repeating unit and phosphorus. Furthermore, we found that both described PS neither induce cytokine production and maturation of mouse BM-DC nor induce signaling through TLR2/TLR4 receptors. However, they differ profoundly in their abilities to modulate the BM-DC immune response to the well-characterized human isolate Lactobacillus plantarum WCFS1. Exposure to L900/2 enhanced interleukin-10 (IL-10) production induced by L. plantarum WCFS1, while in contrast, L900/3 enhanced the production of IL-12p70. We conclude that PS, probably due to their chemical features, are able to modulate the immune responses to third-party antigens. The ability to induce regulatory IL-10 by L900/2 opens up the possibility to use this PS in therapy of inflammatory conditions, such as inflammatory bowel disease, whereas L900/3 might be useful in reverting the antigen-dependent Th2-skewed immune responses in allergies.
Trvalý link: http://hdl.handle.net/11104/0239393