0435478 - BFÚ 2015 RIV NL eng J - Článek v odborném periodiku
Paleček, Emil - Černocká, Hana - Ostatná, Veronika - Navrátilová, Lucie - Brázdová, Marie
Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface.
Analytica Chimica Acta. Roč. 828, MAY2014 (2014), s. 1-8. ISSN 0003-2670. E-ISSN 1873-4324
Grant CEP: GA ČR(CZ) GAP301/11/2055; GA ČR(CZ) GA13-00956S; GA ČR(CZ) GA13-36108S
Institucionální podpora: RVO:68081707
Klíčová slova: Deoxyribonucleic acid-protein binding * Tumor suppressor protein p53 * Electrochemical sensing
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.513, rok: 2014 ; AIS: 1.067, rok: 2014
DOI: https://doi.org/10.1016/j.aca.2014.03.029

Electrochemical biosensors have the unique ability to convert biological events directly into electrical signals suitable for parallel analysis. Here we utilize specific properties of constant current chronopotentiometric stripping (CPS) in the analysis of protein and DNA-protein complex nanolayers. Rapid potential changes at high negative current intensities (I-str) in CPS are utilized in the analysis of DNA-protein interactions at thiol-modified mercury electrodes. P53 core domain (p53CD) sequence-specific binding to DNA results in a striking decrease in the electrocatalytic signal of free p53. This decrease is related to changes in the accessibility of the electroactive amino acid residues in the p53CD-DNA complex. By adjusting I-str and temperature, weaker non-specific binding can be eliminated or distinguished from the sequence-specific binding. The method also reflects differences in the stabilities of different sequence-specific complexes, including those containing spacers between half-sites of the DNA consensus sequence.
Trvalý link: http://hdl.handle.net/11104/0239342