0434569 - FGÚ 2015 RIV DE eng J - Článek v odborném periodiku
Mikšík, Ivan - Ergang, Peter - Pácha, Jiří
Proteomic analysis of chicken eggshell cuticle membrane layer.
Analytical and Bioanalytical Chemistry. Roč. 406, č. 29 (2014), s. 7633-7640. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR(CZ) GA13-17224S
Institucionální podpora: RVO:67985823
Klíčová slova: mass spectrometry * bioanalyticalmethods * cuticle * chicken proteins * insoluble proteins * eggshell
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.436, rok: 2014 ; AIS: 0.91, rok: 2014
DOI: https://doi.org/10.1007/s00216-014-8213-x

The EDTA-insoluble proteinaceous film from the cuticle layer of the eggshell was studied. This film consists of three main areas: spots (cca 300 μm diameter containing pigment), blotches (small spots with diameter only tens of μm), and the surroundings (i.e., the area without spots and blotches). In total, 29 proteins were determined (and another eight by less specific “cleavage” with semitrypsin) but their distribution among various areas of cuticle was inhomogeneous. The most dominant proteins were eggshell-specific ones, ovocleidin-17 and ovocleidin-116
Trvalý link: http://hdl.handle.net/11104/0238570