Počet záznamů: 1  

Molecular Events Accompanying Rous Sarcoma Virus Rescue from Rodent Cells and the Role of Viral Gene Complementation

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    0434387 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
    Lounková, Anna - Dráberová, Eduarda - Šenigl, Filip - Trejbalová, Kateřina - Geryk, Josef - Hejnar, Jiří - Svoboda, Jan
    Molecular Events Accompanying Rous Sarcoma Virus Rescue from Rodent Cells and the Role of Viral Gene Complementation.
    Journal of Virology. Roč. 88, č. 6 (2014), s. 3505-3515. ISSN 0022-538X. E-ISSN 1098-5514
    Grant CEP: GA ČR GAP502/11/2207; GA ČR GAP302/12/1673
    Institucionální podpora: RVO:68378050
    Klíčová slova: Rous sarcoma virus * heterotransmission * cell non-permissiveness
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.439, rok: 2014

    Transformation of rodent cells with avian Rous sarcoma virus (RSV) opened new ways to studying virus integration and expression in nonpermissive cells. We were interested in (i) the molecular changes accompanying fusion of RSV-transformed mammalian cells with avian cells leading to virus rescue and (ii) enhancement of this process by retroviral gene products. The RSV-transformed hamster RSCh cell line was characterized as producing only a marginal amount of env mRNA, no envelope glycoprotein, and a small amount of unprocessed Gag protein. Egress of viral unspliced genomic RNA from the nucleus was hampered, and its stability decreased. Cell fusion of the chicken DF-I cell line with RSCh cells led to production of env mRNA, envelope glycoprotein, and processed Gag and virus-like particle formation. Proteosynthesis inhibition in DF-1 cells suppressed steps leading to virus rescue. Furthermore, new aberrantly spliced env mRNA species were found in the RSCh cells. Finally, we demonstrated that virus rescue efficiency can be significantly increased by complementation with the env gene and the highly expressed gag gene and can be increased the most by a helper virus infection. In summary, Env and Gag synthesis is increased after RSV-transformed hamster cell fusion with chicken fibroblasts, and both proteins provided in trans enhance RSV rescue. We conclude that the chicken fibroblast yields some factor(s) needed for RSV replication, particularly Env and Gag synthesis, in nonpermissive rodent cells.
    Trvalý link: http://hdl.handle.net/11104/0238453

     
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