Počet záznamů: 1  

Comprehensive portrait of cholesterol containing oxidized membrane

  1. 1.
    0427792 - ÚFCH JH 2015 RIV NL eng J - Článek v odborném periodiku
    Štefl, Martin - Šachl, Radek - Olžyńska, Agnieszka - Amaro, Mariana - Savchenko, Dariia - Deyneka, Alexander - Hermetter, A. - Cwiklik, Lukasz - Humpolíčková, Jana - Hof, Martin
    Comprehensive portrait of cholesterol containing oxidized membrane.
    Biochimica Et Biophysica Acta-Biomembranes. Roč. 1838, č. 7 (2014), s. 1769-1776. ISSN 0005-2736. E-ISSN 1879-2642
    Grant CEP: GA ČR GBP208/12/G016; GA MŠk LH13259
    Grant ostatní:SAFMAT(CZ) CZ.2. 13/3.1.00/22132
    Institucionální podpora: RVO:61388955 ; RVO:68378271
    Klíčová slova: oxidized lipids * lateral diffusion * molecular dynamics simulations
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 3.836, rok: 2014

    Biologicalmembranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation,which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
    Trvalý link: http://hdl.handle.net/11104/0233275
     
Počet záznamů: 1