Tailoring morphologies of diamond thin films for neural stem cells culturing

0423240 - FZÚ 2014 AT eng A - Abstrakt
Babchenko, Oleg - Kromka, Alexander - Romanyuk, Nataliya - Jendelová, Pavla
Tailoring morphologies of diamond thin films for neural stem cells culturing.
International Winterschool on Electronic Properties of Novel Materials /27./. Kirchberg: Kirchberg, 2013 - (Machon, M.; Herziger, F.). s. 43-44
[International Winterschool on Electronic Properties of Novel Materials /27./. 02.03.2013-09.03.2013, Kirchberg]
Grant CEP: GA ČR GAP108/12/0910; GA ČR GAP108/12/0996; GA MŠMT(CZ) LM2011026
Institucionální podpora: RVO:68378271 ; RVO:68378041
Klíčová slova: diamond film * neural stem cells * cultivation * structuring
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus

In the case of neural cells or neural stem cells (NSC), NCD possess a great potential as biomaterial for central nervous system transplantation and tissue engineering. In this contribution we investigate influence of oxidized NCD surface morphology with: i) at (as grown), ii) nano-rods and iii) porous structure onto NSCs culturing. Nano-rods (diameter 20-60nm) were fabricated by etching of at NCD lms, while porous structures (random wires diameter below 20 nm) were directly grown at tailored process conditions. We found that nano-rods were not suitable for NSCs. Nevertheless, on at NCD and porous structures the 24-48 hours cells seeding resulted to similar number of attached NSCs. After the 6-days seeding,at NCD were covered by homogeneous NSCs layer, while on samples with porous structure NSCs formed neurospheres, suggesting less preferable conditions for attachment. Further investigations of NSCs to NCD surface adhesion and interaction mechanisms will be discussed.
Trvalý link: http://hdl.handle.net/11104/0229325