Very low energy STEM for biology

0422788 - ÚPT 2014 CZ eng K - Konferenční příspěvek (tuzemská konf.)
Frank, Luděk - Nebesářová, Jana - Vancová, Marie - Paták, Aleš - Müllerová, Ilona
Very low energy STEM for biology.
Mikroskopie 2013. Praha: Československá mikroskopická společnost, 2013, s. 19.
[Mikroskopie 2013. Lednice (CZ), 13.05.2013-14.05.2013]
Grant CEP: GA TA ČR TE01020118; GA ČR GAP108/11/2270; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731 ; RVO:60077344
Klíčová slova: tissue sections * ultra-low-energy STEM * cathode lens
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika

Examination of tissue sections with transmitted electrons has been performed at energies of hundreds and tens of eV with thicknesses of sections of 10 nm or less. This was possible by employing the cathode lens principle working without lowest energy limitations with the help of biasing the sample to a high negative potential. The reflected and transmitted electrons were attracted with the same electric field to earthed detectors situated above and below the sample. Very high image contrasts have been obtained even for samples free of any heavy metal salts for contrast enhancement.
Trvalý link: http://hdl.handle.net/11104/0229236