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Recent advances in the development of single cell analysis-A review

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    0398829 - ÚIACH 2014 RIV NL eng J - Článek v odborném periodiku
    Klepárník, Karel - Foret, František
    Recent advances in the development of single cell analysis-A review.
    Analytica Chimica Acta. Roč. 800, OCT (2013), s. 12-21. ISSN 0003-2670. E-ISSN 1873-4324
    Grant CEP: GA MŠk(CZ) EE2.3.20.0182; GA ČR GAP206/11/2377
    Institucionální podpora: RVO:68081715
    Klíčová slova: single cell analysis * capillary electrophoresis * electrochemistry * microfluidic devices
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 4.517, rok: 2013

    Development of techniques for the analysis of the content of individual cells represents an important direction in modern bioanalytical chemistry. While the analysis of chromosomes, organelles, or location of selected proteins has been traditionally the domain of microscopic techniques, the advances in miniaturized analytical systems bring new possibilities for separations and detections of molecules inside the individual cells including smaller molecules such as hormones or metabolites. It should be stressed that the field of single cell analysis is very broad, covering advanced optical, electrochemical and mass spectrometry instrumentation, sensor technology and separation techniques. The number of papers published on single cell analysis has reached several hundred in recent years. Thus a complete literature coverage is beyond the limits of a journal article. The following text provides a critical overview of some of the latest developments with the main focus on mass spectrometry, microseparation methods, electrophoresis in capillaries and microfluidic devices and respective detection techniques for performing single cell analyses.
    Trvalý link: http://hdl.handle.net/11104/0226228

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