Macroporous cryogels with immobilized enzymes and affinity ligands for glycoprotein analysis

0397635 - ÚIACH 2014 ES eng A - Abstrakt
Křenková, Jana - Foret, František
Macroporous cryogels with immobilized enzymes and affinity ligands for glycoprotein analysis.
20th International Symposium on Electro- and Liquid Phase-separation Techniques. Book of Abstracts. Universidad de La Laguna, 2013. s. 232.
[International Symposium on Electro- and Liquid Phase-separation Techniques /20./. 06.10.2013-09.10.2013, Puerto de la Cruz, Tenerife]
Grant CEP: GA ČR(CZ) GBP206/12/G014; GA ČR(CZ) GAP301/11/2055
Grant ostatní: GA AV ČR(CZ) M200311201
Institucionální podpora: RVO:68081715
Klíčová slova: glycoprotein * cryogel * enzymes * mass spectrometry
Kód oboru RIV: CB - Analytická chemie, separace

In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol)diacrylate) cryogel placed in the centrifugal filter device. The surface of the monolithic scaffold was activated by epichlorohydrin and used for multi-step immobilization of various enzymes and affinity ligands utilized in glycoprotein analysis.
Trvalý link: http://hdl.handle.net/11104/0225248