HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems

0395233 - ÚEM 2014 RIV DE eng J - Článek v odborném periodiku
Fenech, M. - Kirsch-Volders, M. - Rössnerová, Andrea - Šrám, Radim - Romm, H. - Bolognesi, C. - Ramakumar, A. - Soussaline, F. - Schunck, CH. - Elhajouji, A. - Anwar, W. - Bonassi, S.
HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems.
International Journal of Hygiene and Environmental Health. Roč. 216, č. 5 (2013), s. 541-552. ISSN 1438-4639. E-ISSN 1618-131X
Grant CEP: GA ČR GAP503/11/0084
Grant ostatní: Project NewGenesis(XE) FOOD-CT-2005-016320
Institucionální podpora: RVO:68378041
Klíčová slova: Micronucleus * Cytokinesis-block * Automation
Kód oboru RIV: DN - Vliv životního prostředí na zdraví
Impakt faktor: 3.276, rok: 2013

Analysis of micronuclei (MN) is a commonly used method with wide applications. As a consequence there has been a strong interest in developing automated systems to score MN, a biomarker of chromosome breakage or loss, in mammalian and human cells. The review indentifies recent progress, important limitations and steps that need to be taken into account to enable the successful universal implementation of automated micronucleus assays by image cytometry.
Trvalý link: http://hdl.handle.net/11104/0224653