Počet záznamů: 1
Optimized method for identification of the proteomes secreted by cardiac cells
- 1.0392231 - ÚIACH 2014 RIV US eng J - Článek v odborném periodiku
Šťastná, Miroslava - Van Eyk, J.E.
Optimized method for identification of the proteomes secreted by cardiac cells.
Methods in molecular biology. Roč. 1005, č. 1005 (2013), s. 225-235. ISSN 1940-6029
Institucionální podpora: RVO:68081715
Klíčová slova: cardiac cells * secreted proteins * proteomic technology
Kód oboru RIV: CB - Analytická chemie, separace
There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well.
Trvalý link: http://hdl.handle.net/11104/0221151
Název souboru Staženo Velikost Komentář Verze Přístup 0392231_IR.pdf 6 315.2 KB Jiná vyžádat
Počet záznamů: 1