Comparison of germination responses of Anigozanthos flavidus (Haemodoraceae), Gyrostemon racemiger and Gyrostemon ramulosus (Gyrostemonaceae) to smoke-water and the smoke-derived compounds karrikinolide (KAR(1)) and glyceronitrile

0392135 - ÚOCHB 2014 RIV GB eng J - Článek v odborném periodiku
Downes, K. S. - Light, M. E. - Pošta, Martin - Kohout, Ladislav - van Staden, J.
Comparison of germination responses of Anigozanthos flavidus (Haemodoraceae), Gyrostemon racemiger and Gyrostemon ramulosus (Gyrostemonaceae) to smoke-water and the smoke-derived compounds karrikinolide (KAR(1)) and glyceronitrile.
Annals of Botany. Roč. 111, č. 3 (2013), s. 489-497. ISSN 0305-7364. E-ISSN 1095-8290
Institucionální podpora: RVO:61388963
Klíčová slova: butenolide * cyanohydrin * germination * glyceronitrile * karrikinolide
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.295, rok: 2013

Background and Aims A major germination-promoting chemical in smoke-water is 3-methyl-2H-furo[2,3-c]pyran-2-one (karrikinolide, KAR(1)). However, not all species that germinate in response to smoke-water are responsive to KAR(1), such as Tersonia cyathiflora (Gyrostemonaceae). In this study, a test was made of whether two Gyrostemon species (Gyrostemonaceae) that have previously been shown to respond to smoke-water, respond to KAR(1). If not, then the smoke-derived chemical that stimulates germination of these species is currently unknown. Recently, glyceronitrile was isolated from smoke-water and promoted the germination of certain Anigozanthos species (Haemodoraceae). Whether this chemical promotes Gyrostemon racemiger germination is also examined. Furthermore, an investigation was carried out into whether these species germinate in response to smoke-water derived from burning cellulose alone. Methods Gyrostemon racemiger and G. ramulosus seeds were buried after collection and retrieved in autumn the following year when dormancy was alleviated and seeds had become responsive to smoke-water. Anigozanthos flavidus seeds were after-ripened at 35 degrees C to alleviate dormancy. Gyrostemon and Anigozanthos seeds were then tested with 'Seed Starter' smoke-water, KAR(1), glyceronitrile and cellulose-derived smoke-water. Key Results Although Gyrostemon racemiger, G. ramulosus and A. flavidus were all stimulated to germinate by 'Seed Starter' smoke-water, none of these species responded to KAR(1). Gyrostemon racemiger germination was not promoted by glyceronitrile. This is in contrast to A. flavidus, where glyceronitrile, at concentrations of 1-500 mu M, promoted germination, although seedling growth was inhibited at >= 400 mu M. Maximum A. flavidus germination occurred at glyceronitrile concentrations of 25-300 mu M. Some Gyrostemon germination was promoted by cellulose-derived smoke-water.
Trvalý link: http://hdl.handle.net/11104/0221096