The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells

0391930 - ÚMCH 2014 RIV US eng J - Článek v odborném periodiku
Zasońska, Beata Anna - Boiko, N. - Horák, Daniel - Klyuchivska, O. - Macková, Hana - Beneš, Milan J. - Babič, Michal - Trchová, Miroslava - Hromádková, Jiřina - Stoika, R.
The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells.
Journal of Biomedical Nanotechnology. Roč. 9, č. 3 (2013), s. 479-491. ISSN 1550-7033. E-ISSN 1550-7041
Grant CEP: GA ČR GAP206/12/0381; GA MŠMT 7E12053
GRANT EU: European Commission(XE) 246513 - NADINE
Institucionální podpora: RVO:61389013
Klíčová slova: maghemite * core-shell * nanoparticles
Kód oboru RIV: CD - Makromolekulární chemie
Impakt faktor: 7.578, rok: 2013

Gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the “grafting-from“ approach for immobilization of PDMAAm. They included 2,2′-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2′-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated γ-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-γ-Fe2O3 and PDMAAm-ABHA-γ-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat γ-Fe2O3 and PDMAAm-CCHPA-γ-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-γ-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells.
Trvalý link: http://hdl.handle.net/11104/0223353