Počet záznamů: 1  

Efficient disruption of endogenous Bombyx gene by TAL effector nucleases

  1. 1.
    0390514 - BC-A 2013 RIV GB eng J - Článek v odborném periodiku
    Sajwan, Suresh - Takasu, Y. - Tamura, T. - Uchino, K. - Sezutsu, H. - Žurovec, Michal
    Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.
    Insect Biochemistry and Molecular Biology. Roč. 43, č. 1 (2013), s. 17-23. ISSN 0965-1748
    Grant CEP: GA ČR GAP305/10/2406
    Grant ostatní:Japan Society for the Promotion of Science(JP) 23580083
    Institucionální podpora: RVO:60077344
    Klíčová slova: gene targeting * ZFN * nonhomologous end joining
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.420, rok: 2013

    TALENS are engineered nucleases used for targeted gene disruptions in various organisms. We examined the potential of using full length TALENs for site-specific mutagenesis in silkworm. Three full-length TALEN pairs were synthesized by Golden gate assembly, used for activity examination in heterologous yeast system and finally for mutagenesis in Bombyx mori germline. All TALENs were able to generate somatic and germline mutations in silkworms albeit at low frequency.
    Trvalý link: http://hdl.handle.net/11104/0219546