Efficient disruption of endogenous Bombyx gene by TAL effector nucleases

0390514 - BC 2013 RIV GB eng J - Článek v odborném periodiku
Sajwan, Suresh - Takasu, Y. - Tamura, T. - Uchino, K. - Sezutsu, H. - Žurovec, Michal
Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.
Insect Biochemistry and Molecular Biology. Roč. 43, č. 1 (2013), s. 17-23. ISSN 0965-1748. E-ISSN 1879-0240
Grant CEP: GA ČR GAP305/10/2406
Grant ostatní: Japan Society for the Promotion of Science(JP) 23580083
Institucionální podpora: RVO:60077344
Klíčová slova: gene targeting * ZFN * nonhomologous end joining
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.420, rok: 2013
http://www.sciencedirect.com/science/article/pii/S0965174812001622#

TALENS are engineered nucleases used for targeted gene disruptions in various organisms. We examined the potential of using full length TALENs for site-specific mutagenesis in silkworm. Three full-length TALEN pairs were synthesized by Golden gate assembly, used for activity examination in heterologous yeast system and finally for mutagenesis in Bombyx mori germline. All TALENs were able to generate somatic and germline mutations in silkworms albeit at low frequency.
Trvalý link: http://hdl.handle.net/11104/0219546