Počet záznamů: 1  

Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment

  1. 1. 0389865 - BFU-R 2013 RIV GB eng J - Článek v odborném periodiku
    Ogrocká, A. - Sýkorová, Eva - Fajkus, Jiří - Fojtová, M.
    Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment.
    Journal of Experimental Botany. Roč. 63, č. 11 (2012), s. 4233-4241. ISSN 0022-0957
    Institucionální podpora: RVO:68081707
    Klíčová slova: TELOMERASE REVERSE-TRANSCRIPTASE * ARABIDOPSIS-THALIANA * DNA METHYLATION
    Kód oboru RIV: BO - Biofyzika
    Impakt faktor: 5.242, rok: 2012

    Telomerase, an enzyme responsible for the maintenance of linear chromosome ends, is precisely regulated during plant development. In animals, involvement of the epigenetic state of the telomerase reverse transcriptase (TERT) gene in the complex regulation of telomerase activity has been reported. To reveal whether epigenetic mechanisms participate in the regulation of plant telomerase, the relationship between telomerase activity in tissues of Arabidopsis thaliana and DNA methylation and histone modifications in the A. thaliana TERT (AtTERT) upstream region was studied. As expected, a gradual decrease of telomerase activity during leaf maturation was observed. A different pattern with a more progressive loss of telomerase activity and AtTERT transcription during leaf development was revealed in MET1 gene-knockout mutants. Analysis of DNA methylation in the AtTERT upstream region showed low levels of methylated cytosines without notable differences between telomerase-positive and telomerase-negative wild-type tissues.
    Trvalý link: http://hdl.handle.net/11104/0218759