0387334 - ÚIACH 2014 RIV DE eng J - Článek v odborném periodiku
Lišková, Marcela - Klepárník, Karel - Matalová, Eva - Hegrová, Jitka - Přikryl, Jan - Švandová, Eva - Foret, František
Bioluminescence determination of active caspase-3 in single apoptotic cells.
Electrophoresis. Roč. 34, č. 12 (2013), s. 1772-1777. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR GAP206/11/2377
Grant ostatní: GA ČR(CZ) GAP502/12/1285
Program: GA
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: apoptosis * bioluminescence * caspase-3
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.161, rok: 2013 ; AIS: 0.61, rok: 2013
DOI: https://doi.org/10.1002/elps.201200675

In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Trvalý link: http://hdl.handle.net/11104/0218423