Počet záznamů: 1  

Association of the transcription profile of bovine oocytes and embryos with developmental potential

  1. 1.
    0386817 - ÚŽFG 2013 RIV NL eng J - Článek v odborném periodiku
    Kaňka, Jiří - Němcová, Lucie - Toralová, Tereza - Vodičková Kepková, Kateřina - Vodička, Petr - Jeseta, M. - Machatková, M.
    Association of the transcription profile of bovine oocytes and embryos with developmental potential.
    Animal Reproduction Science. Roč. 134, 1-2 (2012), 29-35. ISSN 0378-4320. E-ISSN 1873-2232.
    [Embryo Genomics Meeting /3./. Bonn, 20.08.2012-22.08.2012]
    Grant CEP: GA ČR GA523/09/1035; GA MZe QI91A018
    Institucionální podpora: RVO:67985904
    Klíčová slova: oocyte * in vitro maturation * pre-implantation development
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 1.897, rok: 2012

    Although improvements in culture system have enhanced in vitro embryo production, success rates are still not adequate. The reasons for developmental arrest of a part of in vitro produced embryos are unknown, but are connected in part with low cytoplasmic competence of oocytes. The immaturity of cytoplasm can negatively influence fertilization efficiency and subsequent progression through embryonic genome activation (EGA), which are necessary steps in further pre-implantation development. A large number of studies have compared mRNA abundance among oocytes with different developmental competence with the aim to find markers of the normal embryo development. The amount of mitochondrial DNA (mtDNA) and mRNA for mitochondrial transcriptional factors directing oxidative phosphorylation belongs to such promising markers. Nevertheless, recently published studies revealed that the mammalian embryo is able to compensate for a reduced level of mtDNA in oocyte during subsequent pre-implantation development. The search for other molecular markers is in progress. Characterization of oocyte and embryonic mRNA expression patterns during the pre-implantation period, and their relationship to the successful in vitro and in vivo development will be essential for defining the optimized culture conditions or the nuclear transfer protocols. Microarrays technology enables us to reveal the differentially expressed genes during EGA, and to compare the expression profile of in vivo and in vitro produced embryos. Recent evidence indicates that the depletion of the pool of stored maternal mRNAs is critical for subsequent embryo development. All these experiments gradually offer a list of possible candidates for quality and developmental competence markers for mammalian oocytes and pre-implantation embryos.
    Trvalý link: http://hdl.handle.net/11104/0220207

     
     
Počet záznamů: 1  

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