The efficient approaches for enzymatic digestion of plant proteins

0386005 - ÚIACH 2013 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Dyčka, Filip - Bobáľ, P. - Bobálová, Janette
The efficient approaches for enzymatic digestion of plant proteins.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 267-270. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
Grant CEP: GA MŠMT 1M0570; GA MŠMT 1M06030; GA MŠMT(CZ) EE2.3.20.0182
Institucionální podpora: RVO:68081715
Klíčová slova: ultrasonic * infrared * digestion
Kód oboru RIV: CB - Analytická chemie, separace

In this work the fast and efficient methods applying ultrasound and infrared radiation were employed for in-gel digestion of proteins extracted from Hordeum vulgare. These techniques can be used instead of time consuming conventional protocol prior mass spectrometry analysis. The comparative study indicates that the examined protocols are able to enhance digestion efficiency and protein identification.
Trvalý link: http://hdl.handle.net/11104/0215222