0384624 - ÚEB 2013 RIV GB eng J - Článek v odborném periodiku
Steigerová, J. - Rárová, L. - Oklešťková, Jana - Křížová, K. - Levková, M. - Šváchová, M. - Kolář, Z. - Strnad, Miroslav
Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells.
Food and Chemical Toxicology. Roč. 50, č. 11 (2012), s. 4068-4076. ISSN 0278-6915. E-ISSN 1873-6351
Grant CEP: GA ČR GA301/08/1649
Grant ostatní: GA MŠk(CZ) ED0007/01/01; GA AV ČR(CZ) IAA400550801
Program: ED; IA
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Apoptosis * Brassinosteroids * Cell cycle
Kód oboru RIV: FD - Onkologie a hematologie
Impakt faktor: 3.010, rok: 2012 ; AIS: 0.669, rok: 2012
DOI: https://doi.org/10.1016/j.fct.2012.08.031

Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the anti-proliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G(1) blocks in LNCaP cells accompanied by reductions in cyclin D-1, CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G(2)/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B-1. Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs.
Trvalý link: http://hdl.handle.net/11104/0214206