Počet záznamů: 1  

Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells

  1. 1.
    0384254 - BC 2013 RIV GB eng J - Článek v odborném periodiku
    Chen, G. - Severo, M. S. - Sohail, M. - Sakhon, O. S. - Wikel, S. K. - Kotsyfakis, Michalis - Pedra, J. H. F.
    Ixodes scapularis saliva mitigates inflammatory cytokine secretion during Anaplasma phagocytophilum stimulation of immune cells.
    Parasites & Vectors. Roč. 5, č. 1 (2012), s. 229. ISSN 1756-3305. E-ISSN 1756-3305
    Institucionální podpora: RVO:60077344
    Klíčová slova: Tick * Ixodes scapularis * Saliva * Anaplasma phagocytophilum * Rickettsial agent
    Kód oboru RIV: EC - Imunologie
    Impakt faktor: 3.246, rok: 2012
    http://www.parasitesandvectors.com/content/5/1/229

    BACKGROUND: Ixodes scapularis saliva enables the transmission of infectious agents to the mammalian host due to its immunomodulatory, anesthetic and anti-coagulant properties. However, how I. scapularis saliva influences host cytokine secretion in the presence of the obligate intracellular rickettsial pathogen Anaplasma phagocytophilum remains elusive. METHODS: Bone marrow derived macrophages (BMDMs) were stimulated with pathogen associated molecular patterns (PAMPs) and A. phagocytophilum. Cytokine secretion was measured in the presence and absence of I. scapularis saliva. Human peripheral blood mononuclear cells (PBMCs) were also stimulated with Tumor Necrosis Factor (TNF)-α in the presence and absence of I. scapularis saliva and interleukin (IL)-8 was measured. RESULTS: I. scapularis saliva inhibits inflammatory cytokine secretion by macrophages during stimulation of Toll-like (TLR) and Nod-like receptor (NLR) signaling pathways. The effect of I. scapularis saliva on immune cells is not restricted to murine macrophages because decreasing levels of interleukin (IL)-8 were observed after TNF-α stimulation of human peripheral blood mononuclear cells. I. scapularis saliva also mitigates pro-inflammatory cytokine response by murine macrophages during challenge with A. phagocytophilum. CONCLUSIONS: These findings suggest that I. scapularis may inhibit inflammatory cytokine secretion during rickettsial transmission at the vector-host interface.
    Trvalý link: http://hdl.handle.net/11104/0213962

     
     
Počet záznamů: 1  

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