Simple protein structure-sensitive chronopotentiometric analysis with dithiothreitol-modified Hg electrodes

0383508 - BFÚ 2013 RIV NL eng J - Článek v odborném periodiku
Ostatná, Veronika - Černocká, Hana - Paleček, Emil
Simple protein structure-sensitive chronopotentiometric analysis with dithiothreitol-modified Hg electrodes.
Bioelectrochemistry. Roč. 87, SI (2012), s. 84-88. ISSN 1567-5394. E-ISSN 1878-562X
Grant CEP: GA AV ČR(CZ) KJB100040901; GA ČR(CZ) GAP301/11/2055; GA MŠMT(CZ) LC06035
Výzkumný záměr: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: protein electroanalysis * DTT-modified electrodes * electrocatalysis
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.947, rok: 2012

In the paper the authors studied the properties of the dithiothreitol (DTT) layer at the hanging mercury drop electrode. At higher DTT concentrations, a densely packed pinhole-free layer is formed with the DTT molecules bound to the electrode surface by a single -SH group, oriented perpendicularly to the surface. If a sufficiently high DTT concentration is used, proteins can be co-adsorbed with DTT on liquid Hg or solid amalgam electrodes without the loss of sensitivity for changes in protein structures.
Trvalý link: http://hdl.handle.net/11104/0213421