0383328 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
Li, Z. - H. - Li, P. - Šulc, Miroslav - Hulák, M. - Randák, T.
Hepatic Proteome Sensitivity in Rainbow Trout after Chronically Exposed to a Human Pharmaceutical Verapamil.
Molecular & Cellular Proteomics. Roč. 11, č. 1 (2012), s. 1-7. ISSN 1535-9476. E-ISSN 1535-9484
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: STRESS-RESPONSE * MICROCYSTIN-LR * PROTEINS
Kód oboru RIV: CE - Biochemie
Impakt faktor: 7.251, rok: 2012 ; AIS: 2.784, rok: 2012
DOI: https://doi.org/10.1074/mcp.M111.008409

Verapamil (VRP), a cardiovascular pharmaceutical widely distributed and persistent in the aquatic environment, has potential toxicity to fish and other aquatic organisms. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the protein patterns that are differentially expressed in liver of rainbow trout exposed to sublethal concentrations of VRP (0.5, 27.0, and 270 mu g/liter) for 42 days. Two-dimensional electrophoresis coupled with MALDI-TOF/TOF mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that the expression of six hepatic acidic proteins were markedly altered in the treatment groups compared with the control group; three proteins especially were significantly down-regulated in fish exposed to VRP at environmental related concentration (0.5 mu g/liter)
Trvalý link: http://hdl.handle.net/11104/0213296