0383105 - BC 2013 RIV US eng J - Článek v odborném periodiku
Zhang, L. - Peng, Y. - Wu, S. - Sun, L. - Huang, E. - Huang, T. - Xu, L. - Wu, Ch. - Gelbič, Ivan - Guan, X.
Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed.
Current Microbiology. Roč. 65, č. 6 (2012), s. 784-791. ISSN 0343-8651. E-ISSN 1432-0991
Grant CEP: GA MŠMT 2B08003
Grant ostatní: National Natural Science Foundation of China(CN) 31071745; Ministry of Education of China(CN) 20093515110010; Ministry of Education of China(CN) 20093515120010; Transformation Fund for Agricultural Science and Technology Achievements(CN) 2010GB2C400212; Education Department Foundation(CN) JA12092
Výzkumný záměr: CEZ:AV0Z50070508
Klíčová slova: Bacillus thuringiensis
Kód oboru RIV: EH - Ekologie - společenstva
Impakt faktor: 1.520, rok: 2012 ; AIS: 0.449, rok: 2012
Web výsledku:
http://link.springer.com/content/pdf/10.1007%2Fs00284-012-0231-3
DOI: https://doi.org/10.1007/s00284-012-0231-3

To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry- strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains, the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappeared thereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showed that all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food.
Trvalý link: http://hdl.handle.net/11104/0213151