0382839 - ÚMCH 2013 RIV US eng J - Článek v odborném periodiku
Skálová, Tereza - Kotýnková, Kristýna - Dušková, Jarmila - Hašek, Jindřich - Koval, Tomáš - Kolenko, Petr - Novák, P. - Man, Petr - Hanč, Pavel - Vaněk, Ondřej - Bezouška, K. - Dohnálek, Jan
Mouse Clr-g, a ligand for NK cell activation receptor NKR-P1F: crystal structure and biophysical properties.
Journal of Immunology. Roč. 189, č. 10 (2012), s. 4881-4889. ISSN 0022-1767. E-ISSN 1550-6606
Grant CEP: GA AV ČR KJB101120805; GA ČR GAP302/11/0855; GA ČR GD305/09/H008; GA ČR GA305/07/1073; GA ČR(CZ) GAP207/10/1040; GA MŠMT EE2.3.30.0029; GA ČR GA303/09/0477
GRANT EU: European Commission(XE) 31220 - SPINE2-COMPLEXES
Výzkumný záměr: CEZ:AV0Z40500505; CEZ:AV0Z50200510
Institucionální podpora: RVO:61389013 ; RVO:61388971
Klíčová slova: Clr-g * C-type lectin like fold * NK receptor
Kód oboru RIV: CE - Biochemie; EC - Imunologie (MBU-M)
Impakt faktor: 5.520, rok: 2012 ; AIS: 2.201, rok: 2012
DOI: https://doi.org/10.4049/jimmunol.1200880

Interactions between C-type lectin-like NK cell receptors and their protein ligands form one of the key recognition mechanisms of the innate immune system that is involved in the elimination of cells that have been malignantly transformed, virally infected, or stressed by chemotherapy or other factors. We determined an x-ray structure for the extracellular domain of mouse C-type lectin related (Clr) protein g, a ligand for the activation receptor NKR-P1F. Clr-g forms dimers in the crystal structure resembling those of human CD69. This newly reported structure, together with the previously determined structure of mouse receptor NKR-P1A, allowed the modeling and calculations of electrostatic profiles for other closely related receptors and ligands. Despite the high similarity among Clr-g, Clr-b, and human CD69, these molecules have fundamentally different electrostatics, with distinct polarization of Clr-g. The electrostatic profile of NKR-P1F is complementary to that of Clr-g, which suggests a plausible interaction mechanism based on contacts between surface sites of opposite potential.
Trvalý link: http://hdl.handle.net/11104/0212952