0382747 - MBÚ 2013 RIV GB eng J - Článek v odborném periodiku
Seydlová, G. - Halada, Petr - Fišer, R. - Toman, O. - Ulrych, Aleš - Svobodová, J.
DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress.
Journal of Applied Microbiology. Roč. 112, č. 4 (2012), s. 765-774. ISSN 1364-5072. E-ISSN 1365-2672
Grant CEP: GA MŠMT LC07017
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Bacillus subtilis * ethanol stress * membrane
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.196, rok: 2012

Cytoplasmic membranes of B. subtilis were severely affected by even short-term exposure to 3% (v / v) ethanol: the growth rate and membrane protein synthesis were markedly reduced, and no adaptive alterations in phospholipids were detected. Simultaneously, steady-state DPH fluorescence anisotropy (rss) showed that the membrane rigidity increased substantially. Analysis of the membrane phosphoproteome using in vitro labelling with [c-32 P] ATP revealed the association of DnaK and GroEL chaperones with membrane, indicating a stress induction process. Upon a long-term 3% (v / v) ethanol stress, the cell growth accelerated slightly and the composition of polar head groups and fatty acids of membrane phospholipids underwent an extensive reconstruction. Correspondingly, membrane fluidity turned back to the original rss values of the control cells
Trvalý link: http://hdl.handle.net/11104/0212876