Počet záznamů: 1  

Rapid UPLC-ESI-MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

  1. 1.
    0382519 - ÚEB 2013 RIV US eng J - Článek v odborném periodiku
    Prokudina, E. A. - Havlíček, Libor - Al-Maharik, N. - Lapčík, O. - Strnad, Miroslav - Grúz, Jiří
    Rapid UPLC-ESI-MS/MS method for the analysis of isoflavonoids and other phenylpropanoids.
    Journal of Food Composition and Analysis. Roč. 26, 1-2 (2012), s. 36-42. ISSN 0889-1575. E-ISSN 1096-0481
    Grant CEP: GA AV ČR KAN200380801; GA ČR GA525/09/0994
    Grant ostatní: GA MŠk(CZ) ED0007/01/01; GA ČR(CZ) GPP503/12/P166
    Program: ED; GP
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Isoflavonoids * Isoflavones * Flavonoids
    Kód oboru RIV: EC - Imunologie
    Impakt faktor: 2.088, rok: 2012

    UPLC-ESI-MS/MS method was developed for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A. daidzin, genistin, ononin, sissotrin, osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarine (scopoletin). The analytes were detected in a multiple reaction monitoring mode and quantified using internal standards [C-13(3)]daidzin, [H-2(6)]naringenin and [H-2(4)]genistein. Limits of detection were in the range 0.001-10 pmol injection(-1). The precision of the method (relative standard deviation) ranged from 2.6 to 15.1% and the accuracy was -22.1 to 16.6%. Application of the method was demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and quantified.
    Trvalý link: http://hdl.handle.net/11104/0212719

     
     
Počet záznamů: 1  

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