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LC-ESI-MS/MS Identification of Polar Lipids of Two Thermophilic Anoxybacillus Bacteria Containing a Unique Lipid Pattern

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    0381939 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
    Řezanka, Tomáš - Kambourova, M. - Derekova, A. - Kolouchová, I. - Sigler, Karel
    LC-ESI-MS/MS Identification of Polar Lipids of Two Thermophilic Anoxybacillus Bacteria Containing a Unique Lipid Pattern.
    Lipids. Roč. 47, č. 7 (2012), s. 729-739. ISSN 0024-4201. E-ISSN 1558-9307
    Grant CEP: GA ČR(CZ) GAP503/11/0215
    Institucionální podpora: RVO:61388971
    Klíčová slova: Anoxybacillus * Plasmalogens * O-Aminoacylphosphatidylglycerols
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 2.557, rok: 2012

    Phospholipids and glycolipids from two recently described species belonging to the thermophilic genus Anoxybacillus were analyzed by liquid chromatography-electrospray tandem mass spectrometry (LC/ESIMS/MS). Analysis of total lipids from the facultatively anaerobic A. bogrovensis on a HILIC (Hydrophilic Interaction LIquid Chromatography) column succeeded in separating diacyl-and plasmalogen phospholipids. The LC/ESI-MS/MS analysis of the strict aerobe A. rupiensis revealed the presence of different unique polar lipids, predominantly alanyl-, lysyl-, and glucosyl-phosphatidylglycerols and cardiolipins. Each of the classes of polar lipids was then analyzed by means of the ESI-MS/MS and more than 140 molecular species of six lipid classes from A. bogrovensis and nearly 200 molecular species of nine classes of polar lipids from A. rupiensis were identified. Five classes of unidentified polar lipids were detected in both strains. Plasmalogens were thus determined for the first time in a facultatively anaerobic bacterium, i.e. A. bogrovensis
    Trvalý link: http://hdl.handle.net/11104/0212295

     
     
Počet záznamů: 1  

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