Počet záznamů: 1  

Magnetic porous corn starch for the affinity purifi cation of cyclodextrin glucanotransferase produced by Bacillus circulans

  1. 1.
    0381575 - ÚVGZ 2013 RIV NL eng J - Článek v odborném periodiku
    Šafaříková, Miroslava - Horská, Kateřina - Maděrová, Zdeňka - Tonkova, A. - Ivanova-Pashkoulova, V. - Šafařík, Ivo
    Magnetic porous corn starch for the affinity purifi cation of cyclodextrin glucanotransferase produced by Bacillus circulans.
    Biocatalysis and Biotransformation. Roč. 30, č. 1 (2012), s. 96-101. ISSN 1024-2422. E-ISSN 1029-2446
    Institucionální podpora: RVO:67179843
    Klíčová slova: Bacillus circulans * cyclodextrin glucanotransferase * magnetic starch * magnetic separation
    Kód oboru RIV: EH - Ekologie - společenstva
    Impakt faktor: 0.895, rok: 2012

    Magnetic porous corn starch was prepared as an affi nity adsorbent for the effi cient and simple scale-up procedure for one-step purifi cation of cyclodextrin glucanotransferase (CGTase) from Bacillus circulans . Magnetic affi nity separation enabled isolation of CGTase from cultivation media (volumes between 10 and 400 mL) with ca 60 – 70% recovery after elution with alkaline buffers containing soluble starch; the enzyme purifi cation factor was 19 – 25 in different batches. The majority of ballast proteins were removed during the purifi cation process, which shows high selectivity of the affi nity material used.
    Trvalý link: http://hdl.handle.net/11104/0212011

     
     
Počet záznamů: 1  

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