Počet záznamů: 1  

NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases

  1. 1.
    0381547 - ÚEB 2013 RIV PT eng J - Článek v odborném periodiku
    Potocký, Martin - Pejchar, Přemysl - Gutkowska, Malgorzata - Jiménez-Quesada, M. J. - Potocká, Andrea - Alché, J. - Kost, B. - Žárský, Viktor
    NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases.
    Journal of Plant Physiology. Roč. 169, č. 16 (2012), s. 1654-1663. ISSN 0176-1617. E-ISSN 1618-1328
    Grant CEP: GA ČR GP522/09/P299
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: Pollen tube * Tip growth * NADPH oxidase
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 2.699, rok: 2012

    Reactive oxygen species (ROS) generated by NADPH oxidase (NOX) are crucial for tip growth of pollen tubes. However, the regulation of NOX activity in pollen tubes remains unknown. Using purified plasma membrane fractions from tobacco and olive pollen and tobacco BY-2 cells, we demonstrate that pollen NOX is activated by calcium ions and low abundant signaling phospholipids, such as phosphatidic acid and phosphatidylinositol 4,5-bisphosphate in vitro and in vivo. Our data also suggest possible synergism between Ca2+ and phospholipid-mediated NOX activation in pollen. Rac/Rop small GTPases are also necessary for normal pollen tube growth and have been proposed to regulate ROS production in root hairs. We show here elevated ROS formation in pollen tubes overexpressing wild-type NtRac5 and constitutively active NtRac5, while overexpression of dominant-negative NtRac5 led to a decrease of ROS in pollen tubes. We also show that PA formed by distinct phospholipases D (PLD) is involved in pathways both upstream and downstream of NOX-mediated ROS generation and identify NtPLDδ as a PLD isoform acting in the ROS response pathway.
    Trvalý link: http://hdl.handle.net/11104/0215207

     
     
Počet záznamů: 1  

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