Počet záznamů: 1
Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves
- 1.0381049 - ÚEB 2013 RIV US eng J - Článek v odborném periodiku
Behr, M. - Motyka, Václav - Weihmann, F. - Malbeck, Jiří - Deising, H. B. - Wirsel, S. G. R.
Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves.
Molecular Plant-Microbe Interactions. Roč. 25, č. 8 (2012), s. 1073-1082. ISSN 0894-0282. E-ISSN 1943-7706
Grant CEP: GA ČR(CZ) GAP506/11/0774; GA MŠMT(CZ) LC06034
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Colletotrichum graminicola * maize * cytokinin
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.307, rok: 2012
When inoculated onto maize leaves at the onset of senescence, the hemibiotroph Colletotrichum graminicola causes green islands that are surrounded by senescing tissue. Taking advantage of green islands as indicators of sites of the establishment of successful infection and of advanced high-performance liquid chromatography tandem mass spectrometry methodology, we analyzed changes in the patterns and levels of cytokinins (CK) at high spatial and analytical resolution. Twenty individual CK were detected in green islands. Levels of cis-zeatin-9-riboside and cis-zeatin-9-riboside-5'-monophosphate increased drastically, whereas that of the most prominent CK, cis-zeatin-O-glucoside, decreased. The fungus likely performed these conversions because corresponding activities were also detected in in vitro cultures amended with CK. We found no evidence that C. graminicola is able to synthesize CK entirely de novo in minimal medium but, after adding dimethylallyl diphosphate, a precursor of CK biosynthesis occurring in plants, a series of trans-zeatin isoforms (i.e., trans-zeatin-9-riboside-5'-monophosphate, trans-zeatin-9-riboside, and trans-zeatin) was formed. After applying CK onto uninfected leaves, transcripts of marker genes for senescence, photosynthesis, and assimilate distribution were measured by quantitative reverse-transcribed polymerase chain reaction; furthermore, pulse-amplitude modulation chlorophyll fluorometry and single-photon avalanche diode analyses were conducted. These experiments suggested that modulation of CK metabolism at the infection site affects host physiology.
Trvalý link: http://hdl.handle.net/11104/0211606
Název souboru Staženo Velikost Komentář Verze Přístup 2012_Behr_MOLECULAR PLANT MICROBE INTERACTIONS_1073.pdf 0 710.7 KB Jiná povolen
Počet záznamů: 1