0380685 - ÚEB 2013 RIV IN eng J - Článek v odborném periodiku
Čeřovská, Noemi - Hoffmeisterová, Hana - Moravec, Tomáš - Plchová, Helena - Folwarczna, Jitka - Synková, Helena - Ryšlavá, H. - Ludvíková, V. - Šmahel, M.
Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants.
Journal of Biosciences. Roč. 37, č. 1 (2012), s. 125-133. ISSN 0250-5991. E-ISSN 0973-7138
Grant CEP: GA ČR GA521/06/0973; GA ČR GA521/09/1525
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Human papillomavirus (HPV-16) * L2-and E7-derived epitopes * transient expression
Kód oboru RIV: FD - Onkologie a hematologie
Impakt faktor: 1.759, rok: 2012 ; AIS: 0.627, rok: 2012
DOI: https://doi.org/10.1007/s12038-011-9177-z

Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery.
Trvalý link: http://hdl.handle.net/11104/0211329