0379236 - BÚ 2013 RIV GB eng J - Článek v odborném periodiku
Mikula, Přemysl - Zezulka, Štěpán - Jančula, Daniel - Maršálek, Blahoslav
Metabolic activity and membrane integrity changes in Microcystis aeruginosa – new findings on hydrogen peroxide toxicity in cyanobacteria.
European Journal of Phycology. Roč. 47, č. 3 (2012), s. 195-206. ISSN 0967-0262. E-ISSN 1469-4433
Grant CEP: GA MPO FR-TI3/196; GA TA ČR TA01010356
Institucionální podpora: RVO:67985939
Klíčová slova: hydrogen peroxide * cyanobacteria * flow cytometry
Kód oboru RIV: EF - Botanika
Impakt faktor: 1.912, rok: 2012 ; AIS: 0.68, rok: 2012
DOI: https://doi.org/10.1080/09670262.2012.687144

The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity.
Trvalý link: http://hdl.handle.net/11104/0210491