Silica-coated La.sub.0.75./sub.Sr.sub.0.25./sub.MnO.sub.3./sub. nanoparticles for magnetically driven DNA isolation

0375107 - FZÚ 2012 RIV DE eng J - Článek v odborném periodiku
Trachtová, Š. - Kaman, Ondřej - Španová, A. - Veverka, Pavel - Pollert, Emil - Rittich, B.
Silica-coated La_0.75Sr_0.25MnO₃ nanoparticles for magnetically driven DNA isolation.
Journal of Separation Science. Roč. 34, č. 21 (2011), s. 3077-3082. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA AV ČR KAN200200651
Výzkumný záměr: CEZ:AV0Z10100521
Klíčová slova: DNA isolation * lanthanum manganese perovskite * nanoparticles
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus
Impakt faktor: 2.733, rok: 2011

Magnetic La0.75Sr0.25MnO3 nanoparticles possessing an approximately 20-nm-thick silica shell (LSMO(0.25)@SiO2) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16% PEG 6000–2M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.
Trvalý link: http://hdl.handle.net/11104/0207855