Počet záznamů: 1  

Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms

  1. 1. 0370898 - FGU-C 2012 RIV CZ eng J - Článek v odborném periodiku
    Říčný, Jan - Soukup, Tomáš
    Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms.
    Physiological Research. Roč. 60, č. 6 (2011), s. 899-904 ISSN 0862-8408
    Grant CEP: GA ČR(CZ) GA304/08/0256
    Grant ostatní:EC(XE) LSH-CT-2004-511978
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: muscle fiber types * myosin heavy chains * SDS-PAGE * immunoreactions * thyroid hormones * ELISA
    Kód oboru RIV: EA - Morfologické obory a cytologie
    Impakt faktor: 1.555, rok: 2011

    We have developed a new method for quantitative determination of myosin heavy chain (MyHC) isoforms taking advantage of immunocytochemical differences, based on ELISA principle. We compared compared it with the SDS-PAGE method at the same sample of adult female inbred Lewis strain euthyroid, hyperthyroid and hypothyroid rats. Our results showed that ELISA can be used for a “semi-quantitative” or a “comparative” measurement of MyHC isoforms in multiple muscle samples, but that it is not more exact or faster compared to the SDS-PAGE
    Trvalý link: http://hdl.handle.net/11104/0204573