Počet záznamů: 1  

The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein

  1. 1.
    0370263 - FGÚ 2012 RIV CZ eng J - Článek v odborném periodiku
    Vošahlíková, Miroslava - Svoboda, Petr
    The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein.
    Physiological Research. Roč. 60, č. 3 (2011), s. 541-547. ISSN 0862-8408. E-ISSN 1802-9973
    Grant CEP: GA AV ČR(CZ) IAA500110606; GA MŠMT(CZ) LC554; GA ČR(CZ) GD305/08/H037
    Výzkumný záměr: CEZ:AV0Z50110509
    Klíčová slova: delta-opioid receptor (DOR) * monovalent ions * G(i)1alfa protein
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 1.555, rok: 2011

    The effect of monovalent cations on trimeric G protein Gi1alfa was measured at equimolar concentration of chloride anion in pertussis-toxin (PTX)-treated HEK293 cells stably expressing PTX-insensitive DOR-Gi1alfa (Cys351-Ile351) fusion protein by high-affinity [35S]GTPgamaS binding assay. The high basal level of binding was detected in absence of DOR agonist and monovalent ions and this high level was inhibited with the order of: Naplus je větší než Kplus je větší nez Liplus. The inhibition by monovalent ions was reversed by increasing concentrations of DOR agonist DADLE. The maximum DADLE response was also highest for sodium and decreased in the order of: Naplus je větší než Kplus rovná se Liplus. Our data indicate i) an inherently high activity of trimeric G protein Gi1alfa when expressed within DOR-Gi1alfa fusion protein and determined in the absence of monovalent cations, ii) preferential sensitivity of DOR-Gi1alfa to sodium as far as maximum of agonist response is involved
    Trvalý link: http://hdl.handle.net/11104/0204113

     
     
Počet záznamů: 1  

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