Frequency of aneuploidy related to age in porcine oocytes

0365035 - ÚŽFG 2012 RIV US eng J - Článek v odborném periodiku
Horňák, M. - Jeseta, M. - Musilová, P. - Pavlok, Antonín - Kubelka, Michal - Motlík, Jan - Rubeš, J. - Anger, Martin
Frequency of aneuploidy related to age in porcine oocytes.
PLoS ONE. Roč. 6, č. 4 (2011), s. 1-5. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR GA523/09/0743; GA AV ČR IAA501620801
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: porcine * oocytes * aneuploidy
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 4.092, rok: 2011
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018892

It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent oocytes to study this phenomenon, we have employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), to study the frequency of aneuploidy in porcine oocytes, including rare cells obtained from aged animals. Using this method, we were able to analyze segregation pattern of each individual chromosome during meiosis I. In contrast to the previous reports where conventional methods, such as chromosome spreads or FISH, were used to estimate frequency of aneuploidy, our results presented here show, that the frequency of this phenomenon was overestimated in porcine oocytes.
Trvalý link: http://hdl.handle.net/11104/0200375