Monolithic columns based on a poly(styrene-divinylbenzene-methacrylic acid) copolymer for capillary liquid chromatography of small organic molecules

0358557 - ÚMCH 2012 RIV NL eng J - Článek v odborném periodiku
Svobodová, A. - Křížek, T. - Širc, Jakub - Šálek, Petr - Tesařová, E. - Coufal, P. - Štulík, K.
Monolithic columns based on a poly(styrene-divinylbenzene-methacrylic acid) copolymer for capillary liquid chromatography of small organic molecules.
Journal of Chromatography A. Roč. 1218, č. 11 (2011), s. 1544-1547. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA AV ČR KAN200520804
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: capillary liquid chromatography * methacrylic acid * polystyrene-based monolithic stationary phase
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 4.531, rok: 2011

A very simple and readily performed method is described for the preparation of poly(styrene-divinylbenzene-methacrylic acid) monolithic columns for capillary liquid chromatography. The effect of the methacrylic acid content on the morphological and chromatographic properties has been investigated. Methacrylic acid is shown to be essential for isocratic separations of small organic analytes by capillary liquid chromatography. Column efficiencies of about 28,000 theoretical plates/m have been obtained for all the test compounds. The batch-to-batch and run-to-run repeatability of the retention times is better than 1.5%.
Trvalý link: http://hdl.handle.net/11104/0196558