The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva

0357702 - ÚMG 2011 RIV ES eng J - Článek v odborném periodiku
Merjava, S. - Neuwirth, Aleš - Tanzerova, M. - Jirsová, K.
The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva.
Histology and Histopathology. Roč. 26, č. 3 (2011), s. 323-331. ISSN 0213-3911. E-ISSN 1699-5848
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: Posterior polymorphous dystrophy * Immunohostochemical analysis * Impression cytology
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.480, rok: 2011

The most intense staining present throughout the cornea was observed for CK3, CK5 and CK14; CK19 was found at the corneal periphery only. CK4 and CK10/13 revealed mild to moderate positivity mostly in the superficial layers of the cornea. The suprabasal cell layers of all examined areas showed a strong positivity for CK16. A heterogeneous staining pattern with a centrifugal decrease in the signal was observed for CK8 and CK18. CK5/6, CK14 and CK19 were present in the limbus, where a positive signal for CK3 was observed in the suprabasal and superficial cells only. In contrast to the cornea, CK15 appeared in the basal and suprabasal layers of the limbus. The perilimbal conjunctiva showed strong immunostaining for CK10/13, CK14 and CK19. A moderate signal for CK7 was detected in the superficial layers of the conjunctiva. qRT-PCR confirmed CK6 and CK18 expression in the corneal and conjunctival epithelium.
Trvalý link: http://hdl.handle.net/11104/0195902