The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons

0354390 - MBÚ 2011 RIV US eng J - Článek v odborném periodiku
Chiu, W.-L. - Wagner, Susan - Herrmannová, Anna - Burela, L. - Zhang, F. - Saini, A. K. - Valášek, Leoš - Hinnebusch, A. G.
The C-Terminal Region of Eukaryotic Translation Initiation Factor 3a (eIF3a) Promotes mRNA Recruitment, Scanning, and, Together with eIF3j and the eIF3b RNA Recognition Motif, Selection of AUG Start Codons.
Molecular and Cellular Biology. Roč. 30, č. 18 (2010), s. 4415-4434. ISSN 0270-7306. E-ISSN 1098-5549
Grant CEP: GA ČR GAP305/10/0335
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: 40S RIBOSOMAL-SUBUNIT * SACCHAROMYCES-CEREVISIAE * IN-VIVO
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 6.188, rok: 2010

The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3(eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the mRNA entry channel of the 40S subunit. We have identified substitutions in the conserved Lys-Glu-Arg-Arg (KERR) motif and in residues of the nearby box6 element of the a/Tif32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes indicating defects in scanning and start codon recognition. The normally dispensable CTD of j/Hcr1 is required for its binding to a/Tif32 and to mitigate the growth defects of these a/Tif32 mutants, indicating physical and functional interactions between these two domains
Trvalý link: http://hdl.handle.net/11104/0193401