Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo

0350109 - ÚEM 2011 RIV US eng J - Článek v odborném periodiku
Borghese, L. - Doležalová, Dáša - Opitz, T. - Haupt, S. - Leinhaas, A. - Steinfarz, B. - Koch, P. - Edenhofer, F. - Hampl, Aleš - Brüstle, O.
Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo.
Stem Cells. Roč. 28, č. 5 (2010), s. 955-964. ISSN 1066-5099. E-ISSN 1549-4918
Grant ostatní: GA MŠk(CZ) 1M0538; EC FP6 project ESTOOLS(XE) LSHG-CT-2006-018739; EC FP7 project NeuroStemcell(XE) HEALTH-2007-B-22943
Program: 1M
Výzkumný záměr: CEZ:AV0Z50390703
Klíčová slova: neural stem cells * notch * neuron
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 7.871, rok: 2010
http://arl-repository.lib.cas.cz/uloziste_av/UEM-P/cav_un_epca-0350109_01.pdf

The controlled in vitro differentiation of human embryonic stem cells (hESCs) and other pluripotent stem cells provides interesting prospects for generating large numbers of human neurons for a variety of biomedical applications. A major bottleneck associated with this approach is the long time required for hESC-derived neural cells (hESNSCs) to give rise to functional neuronal progeny. Here we assessed the expression of Notch pathway components in hESNSCs and demonstrate that Notch signaling is active under self-renewing culture conditions. Combined with growth factor withdrawal, inhibition of Notch signaling results in a marked acceleration of differentiation, thereby shortening the time required for the generation of electrophysiologically active hESNSC-derived neurons.
Trvalý link: http://hdl.handle.net/11104/0190195