Počet záznamů: 1  

Ribonucleoprotein localization in mouse oocytes

  1. 1. 0346833 - UMG-J 2011 RIV US eng J - Článek v odborném periodiku
    Flemr, Matyáš - Svoboda, Petr
    Ribonucleoprotein localization in mouse oocytes.
    Methods. Roč. 53, č. 2 (2011), s. 136-141 ISSN 1046-2023
    Grant CEP: GA ČR GA204/09/0085; GA ČR GAP305/10/2215; GA MŠk ME09039
    Výzkumný záměr: CEZ:AV0Z50520514
    Klíčová slova: mouse oocyte * in situ hybridization * immunofluorescence
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 4.011, rok: 2011

    RNA molecules rarely function alone in cells. For most RNAs, their function requires formation of various ribonucleoprotein (RNP) complexes. For example, mRNP composition can determine mRNA localization, translational repression, level of translation or mRNA stability. RNPs are usually studied by biochemical methods. However, biochemical approaches are unsuitable for some model systems, such as mammalian oocytes and early embryos, due to the small amounts that can be obtained for experimental analysis. In such cases, microscopic techniques are often used to learn about RNPs. Here, we present a review of immunostaining, fluorescence in situ hybridization with subcellular resolution and a combination of both, with emphasis on the mouse oocyte and early embryos models. Application of these techniques to whole-mount fixed oocytes and early embryos can provide information about RNP composition and localization with three-dimensional resolution.
    Trvalý link: http://hdl.handle.net/11104/0187752