Heterologous expression of full-length capsid protein of porcine circovirus 2 in Escherichia coli and its potential use for detection of antibodies

0334821 - MBÚ 2010 RIV NL eng J - Článek v odborném periodiku
Marčeková, Zuzana - Psikal, P. - Kosinová, E. - Benada, Oldřich - Šebo, Peter - Bumba, Ladislav
Heterologous expression of full-length capsid protein of porcine circovirus 2 in Escherichia coli and its potential use for detection of antibodies.
Journal of Virological Methods. Roč. 162, 1-2 (2009), s. 133-141. ISSN 0166-0934. E-ISSN 1879-0984
Grant CEP: GA ČR GP310/07/P115; GA MŠMT 2B06161
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: PCV 2 * Porcine circovirus * Capsid protein
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.133, rok: 2009

A capsid protein of porcine circovirus 2 (PCV 2) serves as a diagnostic antigen for the detection of PCV 2-associated disease known as a postweaning multisystemic wasting syndrome (PMWS). In this report, a bacterial expression system was developed for the expression and purification of the full-length PCV 2 capsid (Cap) protein from a codon-optimized cap gene. Replacement of rare arginine codons located at the 5_ end of the cap reading frame with codons optimal for E. coli was found to overcome the poor expression of the viral protein in the prokaryotic system. The Cap protein was purified to greater than 95% homogeneity by using a single cation-exchange chromatography at a yield of 10mg per litre of bacterial culture
Trvalý link: http://hdl.handle.net/11104/0179450