Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces

0327089 - MBÚ 2010 RIV US eng J - Článek v odborném periodiku
Sauerzapfe, B. - Křenek, Karel - Schmiedel, J. - Wakarchuk, W.W. - Pelantová, Helena - Křen, Vladimír - Elling, L.
Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces.
[Chemo-enzymatická synthesa poly-N-acetyllactosaminových (poly-LacNAc) structur a jejich characterizace pro CGL2-galectin-zprostředkovanou vazbu ECM glycoproteinů k biomateriálovým povrchům.]
Glycoconjugate Journal. Roč. 26, č. 2 (2009), s. 141-159. ISSN 0282-0080. E-ISSN 1573-4986
Grant CEP: GA AV ČR IAA400200503; GA MŠMT(CZ) LC06010
Grant ostatní: CZ(CZ) DAAD-AV ČR projekt PPP-D7-CZ 26/04-05D/03/44448
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: chemo-enzymatic sysnthesis * galectin binding * biomaterials
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.500, rok: 2009

Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectinmediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized β-GlcNAc glycosides carrying a linker for controlled coupling onto chemically functionalized surfaces. Then we produced poly-LacNAc structures with defined lengths using human β1,4-galactosyl-transferase-1 and β1,3-N-acetylglucosaminyltransferase from Helicobacter pylori. These compounds were also used for kinetic characterization of glycosyltransferases and lectin binding assays. A mixture of poly-LacNAc-structures covalently coupled to functionalized microtiter plates were identified for best binding to our model galectin His6CGL2

Byla popsána chemo-enzymatická synthesa poly-N-acetyllactosaminových (poly-LacNAc) structur a jejich characterizace pro CGL2-galectin-zprostředkovanou vazbu ECM glycoproteinů k biomateriálovým povrchům
Trvalý link: http://hdl.handle.net/11104/0173988