Y chromosome and vimentin used to trace the fate of allogeneic keratinocytes delivered to the wound by the recombined human/pig skin

0191224 - UMG-J 20010295 RIV CZ eng J - Článek v odborném periodiku
Pokorná, Eva - Brož, L. - Veselý, Pavel - Matoušková, Eva
Y chromosome and vimentin used to trace the fate of allogeneic keratinocytes delivered to the wound by the recombined human/pig skin.
Folia Biologica. Roč. 47, č. 4 (2001), s. 128-134. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA MZd IZ4368; GA MZd NK6126
Klíčová slova: allogeneic keratinocytes * xenodermis * Y-chromosome FISH
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.519, rok: 2001

Allogeneic keratinocytes cultured on cell-free pig dermisstimulate wound healing when applied with the epidermal layerfacing the wound. Male keratinocytes were grafted into donorsites of three female patients. Biopsies were taken on days 4, 6 and 9. The Y-chromosome was found (using the fluorescence .I.in situ ./I.hybridization) in donor sites on day 4 and 6, but not on day 9. Conclusion: Allongeneic keratinocytes temporarily "take" to the wound and are replaced by the patient`s cells after about one week.
Trvalý link: http://hdl.handle.net/11104/0086981