0181555 - UFCH-W 20020187 RIV IE eng J - Článek v odborném periodiku
Stiborová, M. - Šimánek, V. - Frei, E. - Hobza, Pavel - Ulrichová, J.
DNA Adduct Formation from Quaternary Benzo[c]phenanthridine Alkaloids Sanguinarine and Chelerythrine as Revealed by the 32P-postlabeling Technique.
Chemico-Biological Interactions. Roč. 140, - (2002), s. 231-242. ISSN 0009-2797. E-ISSN 1872-7786
Grant CEP: GA ČR GA203/01/0996; GA ČR GA203/00/0633
Výzkumný záměr: CEZ:AV0Z4040901
Klíčová slova: DNA adduct formation * chelerythrine * sanquinarine
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 1.261, rok: 2002

Using the P-32-postlabeling assay, we investigated the ability of quaternary benzo[c]phenanthridine alkaloids, sanguinarine, chelerythrine and fagaronine, to form DNA adducts in vitro. Two enhanced versions of the assay (enrichment by nuclease P1 and 1-butanol extraction) were utilized in the study. Hepatic microsomes of rats pre-treated with beta-naphthoflavone or those of uninduced rats, used as metabolic activators, were incubated in the presence of calf thymus DNA and the alkaloids, with NADPH used as a cofactor. Under these conditions sanguinarine and chelerythrine, but not fagaronine, formed DNA adducts detectable by P-32-postlabeling. DNA adduct formation by both alkaloids was found to be concentration dependent. When analyzing different atomic and bond indices of the C-11-C-12 bond (ring B) in alkaloid molecules we found that fagaronine behaved differently from sanguinarine and chelerythrine. While sanguinarine and chelerythrine showed a preference.
Trvalý link: http://hdl.handle.net/11104/0078106