Počet záznamů: 1  

The Influence of the Thymine C5 Methyl Group on Spontaneous Base Pair Breathing in DNA

  1. 1.
    0181433 - UFCH-W 20020099 RIV US eng J - Článek v odborném periodiku
    Wärmländer, S. - Šponer, Jiří - Leijon, M. - Šponer, Judit E.
    The Influence of the Thymine C5 Methyl Group on Spontaneous Base Pair Breathing in DNA.
    Journal of Biological Chemistry. Roč. 277, č. 32 (2002), s. 28491-28497. ISSN 0021-9258. E-ISSN 1083-351X
    Grant CEP: GA MŠMT LN00A016
    Výzkumný záměr: CEZ:AV0Z4040901
    Klíčová slova: thymine * DNA * base pairs
    Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
    Impakt faktor: 6.696, rok: 2002

    Sequences of four or more AT base pairs without a 5''-TA-3'' step, so-called A-tracts, influence the global properties of DNA by causing curvature of the helix axis if phased with the helical repeat and also influence nucleosome packaging. Hence it is interesting to understand this phenomenon on the molecular level, and numerous studies have been devoted to investigations of dynamical and structural features of A-tract DNA. It was early observed that anomalously slow base pair-opening kinetics were a striking physical property unique to DNA A-tracts (Leroy, J. L., Charretier, E., Kochoyan, M., and Gueron, M. (1988) Biochemistry 27,8894-8898). Furthermore, a strong correlation between DNA curvature and anomalously slow base pair-opening dynamics was found. In the present work it is shown, using imino proton exchange measurements by NMR spectroscopy that the main contribution to the dampening of the base pair-opening fluctuations in A-tracts comes from the C5 methylation.
    Trvalý link: http://hdl.handle.net/11104/0078004

     
     

Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.