0173415 - UEM-P 20010011 RIV US eng J - Článek v odborném periodiku
Melčák, Ivo - Melčáková, Štěpánka - Kopský, Vojtěch - Večeřová, Jaromíra - Raška, Ivan
Prespliceosomal Assembly on Micoinjected Precursor mRNA Takes Place in Nuclear Speckles.
Molecular Biology of the Cell. Roč. 12, - (2001), s. 393-406. ISSN 1059-1524. E-ISSN 1939-4586
Grant CEP: GA ČR GA302/99/0587; GA ČR GA304/00/1481; GA MŠMT VS96129
Výzkumný záměr: CEZ:AV0Z5039906
Klíčová slova: splicing nuclear speckles
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 7.700, rok: 2001

We show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. We conclude that the (pre)spliceosomal complexs on microinjected pre-mRNA are formed inside the speckles. Their targeting into and accumulation in the speckles is a result of the cumulative loading of splicing factors to the pre-mRNA and the complexes formed give rise to the speckled pattern observed.
Trvalý link: http://hdl.handle.net/11104/0070448