0172337 - UEB-Q 20033062 RIV US eng J - Článek v odborném periodiku
Franěk, František - Eckschlager, T. - Hermann, K.
Enhancement of Monoclonal Antibody Production by Lysine-Containing Peptides.
Biotechnology Progress. Roč. 19, č. 1 (2003), s. 169-174. ISSN 8756-7938. E-ISSN 1520-6033
Grant CEP: GA MŠMT OC 844.10
Výzkumný záměr: CEZ:AV0Z5038910; CEZ:MSM 111300005
Klíčová slova: Monoclonal Antibody * Lysine-Containing Peptides
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.488, rok: 2003

In the search for peptides that could effectively enhance the monoclonal antibody production of a model hybridoma, the performance of five lysine-containing peptides was compared. The capacity of the peptides to enhance the monoclonal antibody yield correlated with their growth-suppressing activity. No correlation of the production-enhancing activity with the character of the distribution of cell-cycle phases could be found. All of the tested peptides, including the negative control peptide Gly-Phe-Gly, altered the cell-cycle phases distribution in favor of the proportion of the S phase.The peptides added to the hybridoma culture were found to be gradually decomposed into dipeptides and free amino acids. Among the set of tested lysine-containing di- to pentapeptides, the best results were obtained with the tripeptide Gly-Lys-Gly. The growth-suppressing and production-enhancing capacity of this peptide supplement was obviously associated with the temporary presence of the intact peptide molecule in the culture media, because the addition of a mixture of free amino acids constituting this peptide, i.e., glycine and lysine, displayed a different effect-a slight promotion of cell growth.
Trvalý link: http://hdl.handle.net/11104/0069376